Ectopic activation of the Spindle Assembly Checkpoint reveals its biochemical design and physiological operation
نویسندگان
چکیده
Department of Biophysics, University of Michigan, Ann Arbor, MI 48109 Whitehead Institute for Biomedical Research, and Department of Biology, MIT, Nine Cambridge Center, Cambridge, MA 02142 Department of Biological Sciences, Virginia Polytechnic Institute & State University, Blacksburg, VA 24061 Cell & Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109 *Correspondence to: [email protected] †Present address: Comprehensive Cancer Center, Ohio State University, 460 W. 12th Ave, Columbus, OH 43210.
منابع مشابه
Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption.
The spindle assembly checkpoint keeps cells with defective spindles from initiating chromosome segregation. The protein kinase Mps1 phosphorylates the yeast protein Mad1p when this checkpoint is activated, and the overexpression of Mps1p induces modification of Mad1p and arrests wild-type yeast cells in mitosis with morphologically normal spindles. Spindle assembly checkpoint mutants overexpres...
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The spindle assembly checkpoint (SAC) is a quality control mechanism that ensures accurate chromosome segregation during cell division. It consists of a mechanochemical signal transduction mechanism that senses the attachment of chromosomes to the spindle, and a signaling cascade that inhibits cell division if one or more chromosomes are not attached. Extensive investigations of both these comp...
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The spindle assembly checkpoint prevents cells from initiating anaphase until the spindle has been fully assembled. We previously isolated mitotic arrest deficient (mad) mutants that inactivate this checkpoint and thus increase the sensitivity of cells to benomyl, a drug that interferes with mitotic spindle assembly by depolymerizing microtubules. We have cloned the MAD1 gene and show that when...
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